I used the built in microphone of a laptop computer to record the sound of striking a spoke with a small metal bar.
After damping most of the spokes with a cloth, it sounded like this.
Then I gave the nipple a quarter turn and it sounded like this
I next plotted the spectrum:
The red line is after giving a twist to the nipple.
The fundamental around 600hz is in good agreement with my ear.
The increase from around 580hz to 610hz is easy to see.
I tried this a couple of times with differant spokes and it seems repeatable.
A quick and dirty filter in C or matlab would probably make this a viable method of
measuring relative tension without buying any equipment.